LUMINICELL TRACKERTM – VASCULAR LABELLING KITS
Luminicell TrackerTM are biocompatible organic fluorescent nanoparticles, that can be used to fluorescently tag vasculature in living tissues and animals to visualise vascular structures and study inflammation and vascular leakage. Under normal conditions, these nanoparticles flow smoothly inside blood vessels. However, under inflammation or infection, they leak out in response to blood vessel permeability changes and form localized punctate aggregates, accumulating in surrounding tissues.
Luminicell TrackerTM contains unique organic molecules in the nanoparticle core that possess Aggregation Induced Emission (AIE) properties. Unlike conventional fluorophores, these AIE molecules emit strong fluorescence in aggregate formation at the nanoparticle core. Normal fluorophores suffers from an effect commonly known as aggregation caused quenching (ACQ), which molecules loss fluorescence upon aggregation.
Luminicell TrackerTM is built to be highly compatible with both one-photon and multi-photon fluorescence imaging, enabling deep tissue imaging applications with minimal biological interference. These excellent properties make them optimal candidates to be used as diagnostic reagents.
High Brightness
Photostable
10X brighter fluorescence than quantum dots of similar sizes
Stable intensity after 30 minutes of continuous laser irradiation
Table 1. Compatible instrument parameters for Luminicell Tracker.
* Denotes the best excitation wavelength for fluorescent signal.
TECHNOLOGY
Luminicell TrackerTM is designed and built with patented technology platform that integrates three technology edges:
Scheme 1. Luminicell TrackerTM prioprietary technology platform.
Combining above technology edges resulted in the ultra-brightness, long-lived fluorescent with low cytotoxicity for Luminicell TrackerTM. This allows researchers to perform long-term vascular imaging. Luminicell TrackerTM – Vascular Labelling Kit is designed with inert surface groups, which does not bind specifically to any biomarkers, allowing them to flow smoothly inside blood vessels. Under inflammation or infection, they leak out in response to blood vessel permeability changes form localized punctate aggregates, accumulating at surrounding tissues.
APPLICATIONS
Early identification of haemorrhage sites of mice
Fig 1. Light sheet microscopic whole-brain images of naïve mouse (left) or experimental cerebral malaria (ECM) infected mice. Brain vasculatures for the naïve mouse brain and haemorrhage sites for the ECM mouse brain can be highlighted clearly with Luminicell TrackerTM 670 respectively.
Real-time two-photon blood vasculature imaging in live mice
Fig 2. Models of the mouse brain, ear dermis, and bone marrow, imaged with an intravital two-photon fluorescence microscope, vascular labelled with Luminicell TrackerTM 540.
3D Vascular Reconstruction
Fig 3. 3D reconstruction of vascular systems with clear imaging, labelled with Luminicell TrackerTM 670.
Two-photon absorption spectra of Luminicell Tracker
Fig 4. Two-photon absorption spectra for Luminicell TrackerTM 540 and 670.