LUMINICELL TRACKERTM – CELL LABELLING KITS
Luminicell TrackerTM are biocompatible organic nanoparticles with great biocompatibility, photostability, and high signal intensity. The products are designed to be used for long-term tracking of live cells. Once Luminicell TrackerTM enter the cells, they remain strong and stable fluorescence that allows long-term in vitro tracking of stained cells for up to 10 generations and monitoring the distribution and fate of in vivo transplanted cells for 42 days. These properties make them optimal candidates for long interval live cell bioimaging experiments.
Luminicell TrackerTM contains unique organic molecules in the nanoparticle core that possess aggregation-induced emission (AIE) properties. Unlike conventional fluorophores, these AIE molecules emit strong fluorescence in aggregate form at the nanoparticle core. Normal fluorophores suffer from an effect commonly known as aggregation-caused quenching (ACQ), in which molecules lose fluorescence upon aggregation.
High Brightness
Photostable
Biocompatible
Cell Tracking
10X brighter fluorescence than quantum dots of similar sizes
Stable intensity after 30 minutes of continuous laser irradiation
Stable intensity after 30 minutes of continuous laser irradiation
10 cell generations in vitro, 42 days in vivo
Table 1.
Luminicell Tracker selections and their compatible instrument parameters.
실험프로토콜
* Denotes the best excitation wavelength for fluorescent signal.
TECHNOLOGY
Luminicell TrackerTM is designed and built with patented technology platform that integrates three technology edges:
Scheme 1. Luminicell TrackerTM prioprietary technology platform.
Combining the above technology edges resulted in the ultra-brightness, long-lived fluorescent with low cytotoxicity for Luminicell TrackerTM. This allows researchers to perform long-term live cell tracking, monitoring of cell movement, cell fate, and deep tissue imaging, suitable for in vivo biodistribution studies.
APPLICATIONS
Compatible and efficient cell labelling with different cell lines
Fig 1. Comparative flow cytometry histograms and confocal images of different cell lines transfected with green fluorescent protein (GFP) or labelled with 2 nM of Luminicell TrackerTM 540 overnight at 37 oC. (From left to right: SW480, DLD-1, NCM460, NHDF, HEK293T, BMSCs)
Cell proliferation and lineage tracing of cancer cells
Fig 2. Flow cytometry histogram and confocal images of MCF-7 cells labelled with 2 nM of Luminicell TrackerTM 670 for 4 hours at 37 oC and subcultured for designated passages.
Long-term in vitro tracking of adipose-derived stem cells (ADSCs)
Fig 3. Confocal images of ADSCs labelled with 1 nM of Luminicell TrackerTM 670 (red) for 4 hours at 37 oC and then subcultured for 1 and 5 days, respectively.
Tracking of ADSCsin vivo at single-cell resolution
Fig 4. Confocal images of mice ischemic hind limb slices showing ADSCs labelled with Luminicell TrackerTM 670 (red), post intramuscular injection for (A, B) 30 days and (C) 42 days.
In vivo localization of ADSCs in mice
Fig 5. Mice injected with ADSCs that have been pre-labelled with 1 nM of Luminicell TrackerTM 670 were imaged with in vivo fluorescence imaging system for up to 42 days.
In vivo biodistribution of mesenchymal stem cells (MSCs) in mice
Fig 6. Ex vivo images of organs taken using IVIS Spectrum CT showing the distribution of cells in different organs at 2 weeks. Cells were labelled with Luminicell TrackerTM 670.